1966. ENVIRONMENTAL CLEANING OF EFFLUENT OF SELECTED TEXTILE INDUSTRIES BY BIOSURFACTANT PRODUCING BACTERIA
Invited abstract in session TA-23: Community OR, stream OR for Societal Development.
Tuesday, 8:30-10:00Room: Esther Simpson 3.01
Authors (first author is the speaker)
| 1. | ABIODUN WAIDI ASHIRU
|
| BIOLOGICAL SCIENCE, YABA COLLEGE OF TECHNOLOGY, YABA LAGOS |
Abstract
The aim of this study is to isolate, screen and molecular characterize biosurfactant producing bacteria from effluent of selected textile industries. Effluent samples were collected from ABY textile industrial company, Agege Motor Road, Mushin Lagos state and from Sunflag Nigeria Limited, Surulere Lagos state. Bacteria isolation from the effluents was done by serial dilution and spread plate method on Nutrient agar medium then passes through incubation process for 24 hours for colonies to be present. Screening method used are hemolytic, drop collapse and oilspread techniques to check for biosurfactant present in the effluent samples, which results as positive, negative, gamma, alpha and beta. Gram staining was done to the isolates and viewed under microscopic view showing mostly of Gram positive bacillus and also Gram negative bacillus. Biochemical test was done. Molecular Characterization was done showing, Bacillus tropicus, Lysinibacillus fluoroglycofenilyticus, Paenibacillus sonchi, and Bacillus tianshenii. The PCR analysis of amplified, full length bacterial 16SrRNA fragments from the isolates obtained. The suspected organisms isolated from the samples includes; Bacillus thuringesis, Bacillus subtilis, with different Bacillus spp. The result of the gram staining done from the isolate collected from the textile industries is mostly Gram Positive Bacilli showing the predominant Bacilli present in the effluents from the industries.
Keywords
- Environmental Management
- Water Management
- OR in Sustainability
Status: accepted
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